Publications
Preprints
High-Throughput profiling of SH2 domains using Affimer reagents: unravelling protein interaction networks Sophie J Heseltine, Gregory J Billenness, Heather L Martin, Christian Tiede, Anna AS Tang, Eleanor Foy, Grace Reddy, Naomi Gibson, Michael E Webb, Michael J McPherson, Darren C Tomlinson, Matt Johnson ResearchSquare (2024) link
A ‘glyco-fluorine’ code revealing differential recognition by glycan binding partners Kristian Hollingsworth, Antonio Di Maio, Sarah-Jane Richards, Jean-Baptiste Vendeville, David E. Wheatley, Claire E. Council, Tessa Keenan, Helene Ledru, Harriet Chidwick, Huang Kun, Fabio Parmeggiani, Andrea Marchesi, Wengang Chai, Ryan McBerney, Tomasz P. Kaminski, Matthew R. Balmforth, Alexandra Tamasanu, Simon Charnock, Stuart L. Warriner, Michael E. Webb, Martin A. Fascione, Sabine Flitsch, M. Carmen Galan, Ten Feizi, Matthew I. Gibson, Yan Liu, W. Bruce Turnbull, Bruno Linclau ChemRxiv (2023) link
Sortase-modified cholera toxoids show specific Golgi localization Darren Machin, Daniel Williamson, Peter Fisher, Gemma Wildsmith, James Ross, Christopher Wasson, Andrew MacDonald, Benjamin I Andrews, Daniel Ungar, W Bruce Turnbull, Michael WebbchemRxiv (2019) link
2024
61. The mutagenic plasticity of the Cholera toxin B-subunit surface residues; stability and affinity Cheuk Wang W. Au, Iain W. Manfield, Michael E. Webb, Emanuele Paci, W. Bruce Turnbull, James F. Ross Toxins (Basel) (2024) in press
60. Quantitative N- or C-Terminal Protein Labelling with Unactivated Peptides by Use of Sortases and a D-Aminopeptidase Zoe L.P. Arnott, Holly E. Morgan, Kristian Hollingsworth, Charlotte M. E. Stevenson, Lawrence J. Collins, Alexandra Tamasanu, Darren C. Machin, Jonathan P. Dolan, Tomasz P. Kaminski, Gemma C. Wildsmith, Daniel J. Williamson, Isabelle B. Pickles, Stuart L. Warriner, W. Bruce Turnbull and Michael E. Webb Angew. Chem. Int Ed. (2024) 63, e202310862
2022
59. Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids Sarah Wehrum, Lina Siukstaite, Daniel J Williamson, Thomas R Branson, Taras Sych, Josef Madl, Gemma C Wildsmith, Wenyue Dai, Erik Kempmann, James F Ross, Maren Thomsen, Michael E Webb, Winfried Römer, W Bruce Turnbull ACS Syn. Biol. (2022) 11, 3929-3938
58. Combined application of orthogonal sortases and depsipeptide substrates for dual protein labelling Holly E. Morgan, Zoe L.P. Arnott, Tomasz P. Kaminski, W. Bruce Turnbull & Michael E. Webb Bioconjugate Chem. (2022) 33 2341-2347
57. The N-terminal substrate specificity of the SurE peptide cyclase Asif Fazal, Jake Wheeler, Michael E. Webb and Ryan F. Seipke Org. Biomol. Chem. (2022) 20, 7232-7235
56. Bio-orthogonal, bifunctional linker for engineering synthetic glycoproteins Ryan McBerney, Jonathan P Dolan, Emma E. Cawood, Michael E Webb, W Bruce Turnbull JACS Au (2022) 2, 2038–2047
55. Synthesis of cholera toxin B-subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions Jonathan P Dolan, Darren C Machin, Simone Dedola, Robert A Field, Michael E Webb, W Bruce Turnbull Frontiers in Chemistry (2022) 10 958272
54. In-depth characterization of a re-engineered cholera toxin manufacturing process using growth-decoupled production in Escherichia coli Natalia Danielewicz, Wenyue Dai, Francesca Rosato, Michael Webb, Gerald Striedner, Winfried Römer, W Bruce Turnbull, Juergen Mairhofer Toxins (Basel) (2022) 14 396
53. Challenges in the use of sortase and other peptide ligases for site-specific protein modification Holly E. Morgan, W. Bruce Turnbull and Michael E. Webb Chem. Soc. Rev. (2022) 51 4121-4145
2021
52. Piggybacking on the Cholera Toxin: Identification of a Toxoid-binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons Matthew R Balmforth, Jessica Haigh, Vajinder Kumar, Wenyue Dai, Christian Tiede, Darren C Tomlinson, Jim Deuchars, Michael E Webb, W Bruce TurnbullBioconjug. Chem. (2021) 32 2205-2212
51. A standalone beta-ketoreductase acts concomitantly with biosynthesis of the antimycin scaffold Asif Fazal, Glyn R Hemsworth, Michael E. Webb and Ryan F. Seipke ACS Chemical Biology (2021) 16 1152-1158
2020
50. Biosynthesis of desotamide-type antibiotics Asif Fazal, Michael E. Webb and Ryan F. Seipke Antibiotics (2020) 9 452
49. A versatile cholera toxin conjugate for neuronal targeting and tracing Jessica L Haigh, Daniel J Williamson, Emma Poole, Yuan Guo, Dejian Zhou, Michael E Webb, Susan A Deuchars, Jim Deuchars and W Bruce Turnbull Chem. Commun. (2020) 56, 6098-6501
2019
48. A trans-acting cyclase off-loading strategy for non-ribosomal peptide synthetases Divya Thankachan, Asif Fazal, Daniel Francis, Lijiang Song, Michael E. Webb, and Ryan F. Seipke ACS Chem. Biol. (2019) 14, 845-849
47. A 'catch-and-release' receptor for the Cholera toxin Clare S. Mahon, Gemma C Wildsmith, Diksha Haksar, Eyleen de Poel, Roland Pieters, Jeffrey M Beekman, Michael E Webb and W Bruce Turnbull Faraday Disc. (2019) 219 112-127
46. Directed assembly of homo-pentameric cholera toxin B-subunit proteins into higher-order structures using coiled-coil appendages James F. Ross, Gemma C. Wildsmith, Michael Johnson, Daniel Hurdiss, Kristian Hollingsworth, Rebecca F. Thompson, Majid Mosayebi, Chi H. Trinh, Emanuele Paci, Arwen R. Pearson, Michael E. Webb and W. Bruce Turnbull J. Amer. Chem. Soc. (2019) 141, 5211-5219
45. Control of Conformation in α-Helix Mimicking Aromatic Oligoamide Foldamers Through Interactions Between Adjacent Side-Chains Irene Arrata, Claire M. Grison, Heather Coubrough, Panchami Prabhakaran, Marc Little, Darren C. Tomlinson, Michael E. Webb and Andrew J. Wilson Org. Biomol. Chem. (2019) 17, 3861-3867
44. A Catalytic Protein-Proteomimetic Complex: Using Aromatic Oligoamide Foldamers as Activators of RNase S Zsófia Hegedüs, Claire M. Grison, Jennifer A. Miles, Silvia Rodriguez Marin, Stuart L. Warriner, Michael E. Webb and Andrew J. Wilson Chem. Sci. (2019) 10, 3956-3962
43. Identification of a cyanobacterial aldehyde dehydrogenase that produces retinoic acid in vitro Jennifer A. Miles, Petrinna Machattou, David Nevin-Jones, Michael E. Webb, Andrew Millard, David J. Scanlan and Paul C. Taylor Biochem. Biophys. Res. Commun. (2019) 510 27-34
2018
42. Site-specific Protein Modification and Bio-orthogonal Chemistry Michael E. Webb, Robin S. Bon and Megan H. Wright Chemical and Biological Synthesis 313-356
41. Calorimetric study of substrate binding in individual active sites of bifunctional human ATIC
Danuta Witkowska, Heather L. Cox, Tara C. Hall, Gemma C. Wildsmith, Darren C. Machin, Michael E. Webb Biochem. Biophys. Acta Prot and Proteom. (2018) 1866 254-263
2017
40. Mechanism of regulation of pantothenate biosynthesis by the PanD-PanZ.AcCoA complex reveals an additional mode of action for the antimetabolite N-pentyl pantothenamide (N5-Pan)
Zoe L.P. Arnott, Shingo Nozaki, Diana C.F. Monteiro, Holly E. Morgan, Arwen R. Pearson, Hironori Niki, Michael E. Webb Biochemistry (2017) 56 4931-4939
39. Affimers proteins are versatile and renewable affinity reagents
Christian Tiede, Robert Bedford, Sophie J. Heseltine, Gina Smith, Imeshi Wijetunga, Rebecca L. Ross, Danah Al-Qallaf, Ashley P. Roberts, Alexander Balls, Alistair Curd, Ruth E. Hughes, Heather Martin, Sarah R. Needham, Laura C. Zanetti-Domingues, Yasha Sadigh, Thomas P. Peacock, Anna A. Tang, Naomi Gibson, Hannah Kyle, Geoffrey W. Platt, Nicola Ingram, Thomas W. Taylor, Louise P. Coletta, Iain Manfield, Margaret A. Knowles, Sandra Bell, Filomena Esteves, Azhar Maqbool, Raj J. Prasad, Mark J. Drinkhill, Robin S. Bon, Vikesh Patel, Sarah A. Goodchild, Marisa L. Martin-Fernandez, Ray J. Owens, Joanne E. Nettleship, Michael E. Webb, Michael Harrison, Jonathan D. Lippiat, Sreenivasan Ponnambalam, Michelle Peckham, Alastair Smith, Paul Ko Ferrigno, Matt Johnson, Michael J. McPherson and Darren C. Tomlinson eLife (2017) 24903. See also: Press release
38. Double quick, double click reversible peptide "stapling"
Claire M. Grison, George M. Burslem, Jennifer A. Miles, Ludwig K.A. Pilsl, David J Yeo, Zeynab Imani, Stuart L. Warriner, Michael E. Webb, Andrew J. Wilson Chem. Sci. (2017) 8 5166-5171 See also: press release
37. Protein surface mimetics: understanding how ruthenium tris(bipyridines) interact with proteins
Sarah H Hewitt, Maria H Filby, Ed Hayes, Lars T Kuhn, Arnout P. Kalverda, Michael E Webb and Andrew J Wilson ChemBioChem (2017) 18 223-231
2016
36. The adsorption of fungal ice-nucleating proteins on mineral dusts: a terrestrial reservoir of atmospheric ice-nucleating particles
Daniel O'Sullivan, Benjamin J. Murray, James F. Ross, and Michael E. Webb Atmos. Chem. Phys. (2016) 16 7879-7887
35. Sortase-mediated labelling of lipid nanodiscs for cellular tracing
A. Ivona Petrache, Darren C. Machin, Daniel J. Williamson, Michael E. Webb and Paul A. Beales Mol. Bio. Syst. (2016) 12 1760-1763
34. Confirmation of a protein-protein interaction in the pantothenate biosynthetic pathway using Sortase-mediated labelling
Philip M.Morrison, Matthew R. Balmforth, Samuel W. Ness, Daniel J. Williamson, Michael D. Rugen, W. Bruce Turnbull and Michael E. Webb ChemBioChem (2016) 17 753-758
2015
33. Chemical generation and modification of peptides containing multiple dehydroalanines
Phillip M. Morrison, Patrick J. Foley, Stuart L. Warriner & Michael E. Webb Chem. Commun. (2015) 51 13470-3
32. Strain-promoted reaction of 1,2,4-triazines with bicyclononynes
Katherine A. Horner, Nathalie M. Valette & Michael E. Webb Chem.—Eur. J. (2015) 21 14376-81
31. The Structure of the PanD/PanZ Protein Complex Reveals Negative Feedback Regulation of Pantothenate Biosynthesis by Coenzyme A
Diana C.F. Monteiro, Vijay Patel, Christopher P. Bartlett, Shingo Nozaki, Thomas D. Grant, James A. Gowdy, Gary S. Thompson, Arnout P. Kalverda, Edward H. Snell, Hironori Niki, Arwen R. Pearson & Michael E. Webb Chem. Biol. (2015) 22 492-503
30. The relevance of nanoscale biological fragments for ice nucleation in clouds
Daniel O'Sullivan, Benjamin J. Murray, James F. Ross, Tom F. Whale, Hannah C. Price, James D. Atkinson, Nsikanabasi S. Umo & Michael E Webb Sci. Rep. (2015) 5 8082
2014
29. Evaluation of the interaction between phosphohistidine analogues and phosphotyrosine-binding domains
Tom E McAllister, Katherine A. Horner & Michael E Webb ChemBioChem (2014) 15 1088-1091
28. Threonine 57 is required for the post-translational activation of Escherichia coli aspartate alpha-decarboxylase
Michael E Webb, Briony A Yorke, Tom Kershaw, Sarah Lovelock, Carina M C Lobley, Mairi L Kilkenny, Alison G Smith, Tom L Blundell, Arwen R Pearson and Chris Abell Acta Cryst. (2014) D70 1176-1172
27. Depsipeptide substrates for sortase-mediated N-terminal protein ligation
D. J. Williamson, M. E. Webb & W. B. Turnbull Nat. Protoc. (2014) 9 253-262
26. Ice nucleation by soil dusts: relative importance of mineral dust and biogenic components
D. O'Sullivan, B. J. Murray, T. L. Malkin, T. Whale, N. S. Umo, J. D. Atkinson, H. C. Price, K. J. Baustian, J. Browse, and M. E. Webb Atmos. Chem. Phys. (2014) 4 1853-1867
2013
25. Prospects for stable analogues of phosphohistidine
Tom E McAllister, Jeffrey J Hollins and Michael E Webb Biochem. Soc. Trans. (2013) 41 1072-1077
2012
24. Ice nucleation by particles immersed in supercooled cloud droplets
Benjamin J Murray, Daniel O'Sullivan, James D Atkinson and Michael E Webb Chem. Soc. Rev. (2012) 41 6519-6554
23. Formation of a heterooctameric complex between ADC and its cognate activating factor, PanZ, is CoA-dependent
Diana C.F. Monteiro, Michael D Rugen, Dale Shepherd, Shingo Nozaki, Niki Hironori and Michael E Webb Biochem. Biophys. Res. Commun. (2012) 426 350-355
22. Efficient N-terminal labelling of proteins by use of Sortase
Daniel J. Williamson, Martin A. Fascione, Michael E Webb and W. Bruce Turnbull Angew. Chem. (2012) 51 9377-9380
21. An activator for pyruvoyl-dependent L-aspartate alpha-decarboxylase is conserved in a small group of the gamma-proteobacteria including Escherichia coli
Shingo Nozaki, Michael E Webb and Niki Hironori MicrobiologyOpen (2012) 1 298-300
20. Triazole phosphohistidine analogues compatible with the Fmoc-strategy
Tom E. McAllister and Michael E Webb. Org. Biomol. Chem. (2012) 10 4043-4049
19. Characterisation of the evolutionarily conserved iron sulphur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana
Kaushik Saha, Michael E Webb, Stephen EJ Rigby, Helen K Leech, Martin J Warren and Alison G Smith Biochem. J. (2012) 444 227-237
18. The crystal structure of Escherichia coli aspartate alpha-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation.
Michael E Webb, Carina M C Lobley, Fatima Soliman, Mairi L Kilkenny, Alison G Smith, Tom L Blundell and Chris Abell Acta Cryst.(2012) F68 414-417
2011
17. Small-molecule ligands strongly affect the Förster resonance energy transfer between quantum dot and fluorescent protein
Yue Zhang, Haiyan Zhang, Jeff Hollins, Michael E. Webb and Dejian Zhou. Phys. Chem. Chem. Phys. (2011) 13 19427-19436
16. The pyruvate,orthophosphate dikinase regulatory proteins of Arabidopsis are both bifunctional and interact with the catalytic and nucleotide binding domains of pyruvate,orthophosphate dikinase
Holly M. Astley, Kate Parsley, Sylvain Aubry, Chris J. Chastain, Jim N. Burnell, Michael E. Webb and Julian M. Hibberd Plant J. (2011) 68 1070-1080
15. Pantothenate biosynthesis in higher plants
Michael E Webb and Alison G Smith. Adv. Botan. Res. (2011), 58, 203-255
14. Multilayer enzyme-coupled magnetic nanoparticles as efficient, reusable biocatalysts and biosensors.
Josep Garcia, Yue Zhang, Hannah Taylor, Oscar Cespedes, Michael E. Webb and Dejian Zhou. Nanoscale (2011) 3, 3721-3730
13. Fmoc-chemistry of stable analogues of phosphohistidine.
Tom E McAllister, Michael G Nix and Michael E Webb Chem. Commun. (2011) 47, 1297-1299
2009
12. Chlorophyll and folate: intimate link revealed by drug treatment. Michael E. Webb and Alison G. Smith, New Phytologist 2009, 182 (1), 3-5.
11. Oligobenzamide proteomimetic inhibitors of the p53-hDM2 protein-protein interaction.
Jeffrey P Plante, Thomas Burnley, Barbora Malkova, Michael E Webb, Stuart L Warriner, Thomas A Edwards and Andrew J Wilson Chem. Commun. (2009) 5091
Publications prior to 2007
10. Riboswitches regulate thiamine biosynthesis in the model green alga Chlamydomonas reinhardtii
Martin T Croft, Michael Moulin, Michael E Webb and Alison G Smith Proc. Natl. Acad. Sci. U. S. A. (2007) 104 20770-20775
9. Plants need their vitamins too
Alison G. Smith, Martin T. Croft, Michael L. Moulin and Michael E. Webb Curr. Opin. Plant Biol. (2007) 10 266-275
8. Roles of vitamins B5, B9, B12 and molybdenum cofactor at cellular and organismal levels
F. Rébeillé, S. Ravanel. A. Marquet, R. R. Mendel, M.E. Webb, A. G. Smith and M. J. Warren Nat. Prod. Rep. (2007) 24 949-962
7. Elucidating biosynthesis pathways for vitamins and cofactors
M. E. Webb, A. Marquet, R. R. Mendel, F. Rébeillé and A. G. Smith Nat. Prod. Rep. (2007) 24 988-1088
6. Synthesis and biological evaluation of N-{4-[5-(2, 4-diamino-6-oxo-1, 6-dihydropyrimidin-5-yl)-2-(2, 2, 2-trifluoroacetyl) pentyl] benzoyl}-L-glutamic acid as a potential inhibitor of GAR Tfase and the de novo purine biosynthetic pathway
Heng Cheng, Inkyu Hwang, Youhoon Chong, Ali Tavassoli, Michael E Webb, Yan Zhang, Ian A Wilson, Stephen J Benkovic, Dale L Boger Bioorg. Med. Chem. (2005) 13 2592-2599
5. Biosynthesis of pantothenate
Michael E. Webb, Alison G. Smith and Chris Abell Nat. Prod. Rep. (2004) 21 695-721
4. Structural constraints on protein self‐processing in L‐aspartate‐α‐decarboxylase
Florian Schmitzberger, Mairi L Kilkenny, Carina Lobley, Michael E Webb, Mladen Vinkovic, Dijana Matak‐Vinkovic, Michael Witty, Dimitri Y Chirgadze, Alison G Smith, Chris Abell, Tom L Blundell EMBO J. (2003) 22 6193-6204
3. Structural insights into the evolution of the pantothenate-biosynthesis pathway
CMC Lobley, F Schmitzberger, ML Kilkenny, H Whitney, HH Ottenhof, E Chakauya, ME Webb, LM Birch, KL Tuck, Chris Abell, AG Smith, TL Blundell Biochem. Soc. Trans. (2003) 31 563-572
2. Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites
Michael E. Webb Elaine Stephens, Alison G. Smith and Chris Abell Chem. Commun. (2003) 2416-2417
1. Identification of Tyr58 as the proton donor in the aspartate-α-decarboxylase reaction
S Adrian Saldanha, Louise M Birch, Michael E Webb, Brent K Nabbs, Frank von Delft, Alison G Smith, Chris Abell Chem. Commun. (2001) 18 1760-1761